We offer full support for any HT Cell Based Screening project (based on RNAi, CRISPR or compounds) using phenotypic cell-based readouts such as fluorescent or luminescent reporters or immunostaining. All assays are performed in either 96- or 384-well mictrotitre plate format in an automated fashion. We gladly work with researchers on assay set-up, optimization and the development of new techniques. For data analysis, we have a number of software options available in order to extract the maximum amount of data. Statistically verified data will be provided in Excel format.
Apart from the actual screening process, we offer our expertise in assay development, data analysis, statistical analysis and data management, so users will be guided all the way through their screening projects.
For the screening process we use the Hamilton Microlab STAR, Microlab STARlet liquid handling stations and the INCell Analyzer 2200 automated microscope and Synergy HTX Multimode reader for HT readouts. These instruments can also be used separately with the support from the facility. For more information about the instruments, please see Screening Instrumentation.