Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor. / Rafaeva, Maria; Horton, Edward R.; Jensen, Adina R.D.; Madsen, Chris D.; Reuten, Raphael; Willacy, Oliver; Brøchner, Christian B.; Jensen, Thomas H.; Zornhagen, Kamilla Westarp; Crespo, Marina; Grønseth, Dina S.; Nielsen, Sebastian R.; Idorn, Manja; Straten, Per thor; Rohrberg, Kristoffer; Spanggaard, Iben; Højgaard, Martin; Lassen, Ulrik; Erler, Janine T.; Mayorca-Guiliani, Alejandro E.

In: Advanced Healthcare Materials, Vol. 11, No. 1, 2100684, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rafaeva, M, Horton, ER, Jensen, ARD, Madsen, CD, Reuten, R, Willacy, O, Brøchner, CB, Jensen, TH, Zornhagen, KW, Crespo, M, Grønseth, DS, Nielsen, SR, Idorn, M, Straten, PT, Rohrberg, K, Spanggaard, I, Højgaard, M, Lassen, U, Erler, JT & Mayorca-Guiliani, AE 2022, 'Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor', Advanced Healthcare Materials, vol. 11, no. 1, 2100684. https://doi.org/10.1002/adhm.202100684

APA

Rafaeva, M., Horton, E. R., Jensen, A. R. D., Madsen, C. D., Reuten, R., Willacy, O., Brøchner, C. B., Jensen, T. H., Zornhagen, K. W., Crespo, M., Grønseth, D. S., Nielsen, S. R., Idorn, M., Straten, P. T., Rohrberg, K., Spanggaard, I., Højgaard, M., Lassen, U., Erler, J. T., & Mayorca-Guiliani, A. E. (2022). Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor. Advanced Healthcare Materials, 11(1), [2100684]. https://doi.org/10.1002/adhm.202100684

Vancouver

Rafaeva M, Horton ER, Jensen ARD, Madsen CD, Reuten R, Willacy O et al. Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor. Advanced Healthcare Materials. 2022;11(1). 2100684. https://doi.org/10.1002/adhm.202100684

Author

Rafaeva, Maria ; Horton, Edward R. ; Jensen, Adina R.D. ; Madsen, Chris D. ; Reuten, Raphael ; Willacy, Oliver ; Brøchner, Christian B. ; Jensen, Thomas H. ; Zornhagen, Kamilla Westarp ; Crespo, Marina ; Grønseth, Dina S. ; Nielsen, Sebastian R. ; Idorn, Manja ; Straten, Per thor ; Rohrberg, Kristoffer ; Spanggaard, Iben ; Højgaard, Martin ; Lassen, Ulrik ; Erler, Janine T. ; Mayorca-Guiliani, Alejandro E. / Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor. In: Advanced Healthcare Materials. 2022 ; Vol. 11, No. 1.

Bibtex

@article{740c83238d0142cd8273a493b64e4660,

title = "Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor",

abstract = "Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.",

keywords = "cancer metastasis, experimental methods, extracellular matrix, specialized bioreactors",

author = "Maria Rafaeva and Horton, {Edward R.} and Jensen, {Adina R.D.} and Madsen, {Chris D.} and Raphael Reuten and Oliver Willacy and Br{\o}chner, {Christian B.} and Jensen, {Thomas H.} and Zornhagen, {Kamilla Westarp} and Marina Crespo and Gr{\o}nseth, {Dina S.} and Nielsen, {Sebastian R.} and Manja Idorn and Straten, {Per thor} and Kristoffer Rohrberg and Iben Spanggaard and Martin H{\o}jgaard and Ulrik Lassen and Erler, {Janine T.} and Mayorca-Guiliani, {Alejandro E.}",

note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH",

year = "2022",

doi = "10.1002/adhm.202100684",

language = "English",

volume = "11",

journal = "Advanced healthcare materials",

issn = "2192-2640",

publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",

number = "1",

}

RIS

TY - JOUR

T1 - Modeling Metastatic Colonization in a Decellularized Organ Scaffold-Based Perfusion Bioreactor

AU - Rafaeva, Maria

AU - Horton, Edward R.

AU - Jensen, Adina R.D.

AU - Madsen, Chris D.

AU - Reuten, Raphael

AU - Willacy, Oliver

AU - Brøchner, Christian B.

AU - Jensen, Thomas H.

AU - Zornhagen, Kamilla Westarp

AU - Crespo, Marina

AU - Grønseth, Dina S.

AU - Nielsen, Sebastian R.

AU - Idorn, Manja

AU - Straten, Per thor

AU - Rohrberg, Kristoffer

AU - Spanggaard, Iben

AU - Højgaard, Martin

AU - Lassen, Ulrik

AU - Erler, Janine T.

AU - Mayorca-Guiliani, Alejandro E.

PY - 2022

Y1 - 2022

N2 - Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.

AB - Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), a network of proteins and proteoglycans underpinning all tissues, and a critical regulator of homeostasis and disease. However, there is a major lack in tools to study cancer cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused and populated with cells that are challenged to colonize it, is presented. Using a specialized bioreactor chamber, it is possible to monitor cell behavior microscopically (e.g., proliferation, migration) within the organ scaffold. Cancer cells in this system recapitulate cell signaling observed in vivo and remodel complex native ECM. Moreover, the bioreactors are compatible with co-culturing cell types of different genetic origin comprising the normal and tumor microenvironment. This degree of experimental flexibility in an organ-specific and 3D context, opens new possibilities to study cell–cell and cell–ECM interplay and to model diseases in a controllable organ-specific system ex vivo.

KW - cancer metastasis

KW - experimental methods

KW - extracellular matrix

KW - specialized bioreactors

U2 - 10.1002/adhm.202100684

DO - 10.1002/adhm.202100684

M3 - Journal article

C2 - 34734500

AN - SCOPUS:85119064261

VL - 11

JO - Advanced healthcare materials

JF - Advanced healthcare materials

SN - 2192-2640

IS - 1

M1 - 2100684

ER -

ID: 285312199

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