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YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration

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YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. / Yui, Shiro; Azzolin, Luca; Maimets, Martti; Pedersen, Marianne Terndrup; Fordham, Robert P.; Hansen, Stine L.; Larsen, Hjalte L.; Guiu, Jordi; Alves, Mariana R.P.; Rundsten, Carsten F.; Johansen, Jens V.; Li, Yuan; Madsen, Chris D.; Nakamura, Tetsuya; Watanabe, Mamoru; Nielsen, Ole H.; Schweiger, Pawel J.; Piccolo, Stefano; Jensen, Kim B.

In: Cell Stem Cell, Vol. 22, No. 1, 01.01.2018, p. 35-49. e7.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Yui, S, Azzolin, L, Maimets, M, Pedersen, MT, Fordham, RP, Hansen, SL, Larsen, HL, Guiu, J, Alves, MRP, Rundsten, CF, Johansen, JV, Li, Y, Madsen, CD, Nakamura, T, Watanabe, M, Nielsen, OH, Schweiger, PJ, Piccolo, S & Jensen, KB 2018, 'YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration' Cell Stem Cell, vol. 22, no. 1, pp. 35-49. e7. https://doi.org/10.1016/j.stem.2017.11.001

APA

Yui, S., Azzolin, L., Maimets, M., Pedersen, M. T., Fordham, R. P., Hansen, S. L., ... Jensen, K. B. (2018). YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell, 22(1), 35-49. e7. https://doi.org/10.1016/j.stem.2017.11.001

Vancouver

Yui S, Azzolin L, Maimets M, Pedersen MT, Fordham RP, Hansen SL et al. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. Cell Stem Cell. 2018 Jan 1;22(1):35-49. e7. https://doi.org/10.1016/j.stem.2017.11.001

Author

Yui, Shiro ; Azzolin, Luca ; Maimets, Martti ; Pedersen, Marianne Terndrup ; Fordham, Robert P. ; Hansen, Stine L. ; Larsen, Hjalte L. ; Guiu, Jordi ; Alves, Mariana R.P. ; Rundsten, Carsten F. ; Johansen, Jens V. ; Li, Yuan ; Madsen, Chris D. ; Nakamura, Tetsuya ; Watanabe, Mamoru ; Nielsen, Ole H. ; Schweiger, Pawel J. ; Piccolo, Stefano ; Jensen, Kim B. / YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration. In: Cell Stem Cell. 2018 ; Vol. 22, No. 1. pp. 35-49. e7.

Bibtex

@article{49ee823e7c4244c787fde989e08f2a5b,
title = "YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration",
abstract = "Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element. The mechanism that governs tissue regeneration following severe damage to the colonic epithelium remains poorly understood. Jensen and colleagues show that the colonic epithelium undergoes a profound reprogramming into a more primitive state with fetal-like properties. Moreover, they demonstrate that YAP and TAZ operate as essential mechano-sensors during tissue reprogramming.",
keywords = "Intestinal stem cells, Mechano-sensing, Regeneration, Reprogramming, YAP/TAZ",
author = "Shiro Yui and Luca Azzolin and Martti Maimets and Pedersen, {Marianne Terndrup} and Fordham, {Robert P.} and Hansen, {Stine L.} and Larsen, {Hjalte L.} and Jordi Guiu and Alves, {Mariana R.P.} and Rundsten, {Carsten F.} and Johansen, {Jens V.} and Yuan Li and Madsen, {Chris D.} and Tetsuya Nakamura and Mamoru Watanabe and Nielsen, {Ole H.} and Schweiger, {Pawel J.} and Stefano Piccolo and Jensen, {Kim B.}",
year = "2018",
month = "1",
day = "1",
doi = "10.1016/j.stem.2017.11.001",
language = "English",
volume = "22",
pages = "35--49. e7",
journal = "Cell Stem Cell",
issn = "1934-5909",
publisher = "Cell Press",
number = "1",

}

RIS

TY - JOUR

T1 - YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration

AU - Yui, Shiro

AU - Azzolin, Luca

AU - Maimets, Martti

AU - Pedersen, Marianne Terndrup

AU - Fordham, Robert P.

AU - Hansen, Stine L.

AU - Larsen, Hjalte L.

AU - Guiu, Jordi

AU - Alves, Mariana R.P.

AU - Rundsten, Carsten F.

AU - Johansen, Jens V.

AU - Li, Yuan

AU - Madsen, Chris D.

AU - Nakamura, Tetsuya

AU - Watanabe, Mamoru

AU - Nielsen, Ole H.

AU - Schweiger, Pawel J.

AU - Piccolo, Stefano

AU - Jensen, Kim B.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element. The mechanism that governs tissue regeneration following severe damage to the colonic epithelium remains poorly understood. Jensen and colleagues show that the colonic epithelium undergoes a profound reprogramming into a more primitive state with fetal-like properties. Moreover, they demonstrate that YAP and TAZ operate as essential mechano-sensors during tissue reprogramming.

AB - Tissue regeneration requires dynamic cellular adaptation to the wound environment. It is currently unclear how this is orchestrated at the cellular level and how cell fate is affected by severe tissue damage. Here we dissect cell fate transitions during colonic regeneration in a mouse dextran sulfate sodium (DSS) colitis model, and we demonstrate that the epithelium is transiently reprogrammed into a primitive state. This is characterized by de novo expression of fetal markers as well as suppression of markers for adult stem and differentiated cells. The fate change is orchestrated by remodeling the extracellular matrix (ECM), increased FAK/Src signaling, and ultimately YAP/TAZ activation. In a defined cell culture system recapitulating the extracellular matrix remodeling observed in vivo, we show that a collagen 3D matrix supplemented with Wnt ligands is sufficient to sustain endogenous YAP/TAZ and induce conversion of cell fate. This provides a simple model for tissue regeneration, implicating cellular reprogramming as an essential element. The mechanism that governs tissue regeneration following severe damage to the colonic epithelium remains poorly understood. Jensen and colleagues show that the colonic epithelium undergoes a profound reprogramming into a more primitive state with fetal-like properties. Moreover, they demonstrate that YAP and TAZ operate as essential mechano-sensors during tissue reprogramming.

KW - Intestinal stem cells

KW - Mechano-sensing

KW - Regeneration

KW - Reprogramming

KW - YAP/TAZ

U2 - 10.1016/j.stem.2017.11.001

DO - 10.1016/j.stem.2017.11.001

M3 - Journal article

VL - 22

SP - 35-49. e7

JO - Cell Stem Cell

JF - Cell Stem Cell

SN - 1934-5909

IS - 1

ER -

ID: 188113397