Sequencing

The facility is equipped with both short-read and long-read sequencing platforms.

We use the Illumina MiSeq and NextSeq 500 systems for high-quality short-read sequencing. The MiSeq is well-suited for smaller projects such as targeted or amplicon sequencing, while the NextSeq 500 offers higher throughput for larger-scale projects.

Additionally, we have the latest Oxford Nanopore Technologies equipment, including the PromethION P24 and MinION sequencers. These are used for long-read sequencing, methylation analysis, structural variation detection, direct RNA sequencing, and many other applications.

Users are responsible for preparing their sequencing libraries, which must be accurately quantified before sequencing.

All sequencing instruments must be booked through the PPMS (Project Planning and Management System), which is also used to order approved sequencing kits. Remember that only trained super-users can operate the sequencers; training can be arranged upon request.

Illumina sequencing data is securely uploaded to the Illumina BaseSpace Sequence Hub, ready for download and analysis. Oxford Nanopore data can be transferred to the new BRIC-HPC or another external server, pending approval from the facility managers.