Visualization and quantitation of poly A+-mRNA by using in situ hybridization with biotinylated oligonucleotides.
Research output: Contribution to journal › Journal article › Research › peer-review
HeLa cells and synovial tissue sections were used for visualizing poly A+-mRNA after in situ hybridization. We describe a method to biotinylate hybridization probes by tailing oligo-dT nucleotides with biotinyl-dUTP using terminal deoxynucleotidyl transferase (E.C.2.7.7.3.1.). The hybrids were detected by using the strepavidin-biotin-peroxidase complex (ABC method). The reaction product of the peroxidase staining in the cells and in the tissue sections, is quantitated by the method of plug- and scanning photometry respectively.
Original language | English |
---|---|
Journal | Acta histochemica. Supplementband |
Volume | 37 |
Pages (from-to) | 83-8 |
Number of pages | 5 |
ISSN | 0567-7556 |
Publication status | Published - 1989 |
Externally published | Yes |
Bibliographical note
Keywords: Hela Cells; Humans; Immunoenzyme Techniques; Neoplasm Proteins; Nucleic Acid Hybridization; Oligonucleotide Probes; Poly A; RNA, Messenger
ID: 5240525