To maximize the effectiveness of our collaboration and precisely evaluate your project requirements and associated costs, we kindly ask you to provide the following preliminary information.
Laboratory Automation Screening and Microscopy (LASM)
BRIC’s Laboratory Automation Screening and Microscopy Facility offers assay automation, high content screening, high throughput flow cytometry, MACSima, and access to a wide variety of light microscopy machines for both live and fixed cell imaging.
The facility is fully open to researchers in academia as well as external and industrial researchers.
BRIC’s Core Facility for Laboratory Automation Screening and Microscopy (LASM) offers cutting-edge assay automation, imaging, and cell-based screening services.
Our open-access, flexible, state-of-the-art screening platform unlocks a wide array of possibilities for automated assays, including:
- Arrayed CRISPR knockout and CRISPRa libraries (can be cherry picked)
- Compound Library and screening
- 2D and 3D Cell Culture Assays
- Automated and miniaturized multi-well plate assays
For all these assays and more, we offer readouts in
- High-Throughput Confocal Microscopy
- High-Throughput Live Cell Imaging
- High-Throughput Flow Cytometry
- Luminescence, fluorescence and absorbance measurements
Our team is dedicated to supporting you at every step of your research journey, offering assistance with:
- Initial assay setup
- Automation of assays
- Screen performance
- Data and statistical analysis
- Candidate validation
Explore the full potential of your research with our comprehensive support and advanced technologies. Join us and take advantage of our resources to drive your discoveries forward!
Service
- 500 DKK/hour Academic
- 1000 DKK/hour Industrial
Instrument usage
Working hours (8am-4pm) |
Academic |
Industrial |
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STARlet |
100 DKK/hour |
400 DKK/hour |
| ImageXpress Confocal HT.ai |
250 DKK/hour |
500 DKK/hour |
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Echo 550 and 525 |
200 DKK/hour |
420 DKK/hour |
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IncuCyte S3 |
10 DKK/hour and plate |
40 DKK/hour and plate |
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EnSight Multimode plate reader |
50 DKK/hour |
150 DKK/hour |
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MultiFloFx |
90 DKK/hour |
150 DKK/hour |
| Image analysis computer |
60 DKK/hour |
150 DKK/hour |
| Analysis computer with external |
60 DKK/hour |
150 DKK/hour |
| iQue |
250 DKK/hour |
500 DKK/hour |
Libraries
Genome wide human single guide RNA (sgRNA) library from Horizon targeting 19035 genes
Cherry picked customized sublibraries can be purchased in 384-well format.
Microscopes
Fast, sensitive and flexible automated spinning disk confocal cell imaging using 4x, 10x, 20x (water immersion) or 40x (water immersion) objectives and filters for imaging DAPI, CFP, FITC, YFP, TRITC, Cy5, Cy7 and TexasRed.
Broad range of high content assays in 2D and 3D, can be run on the automation platform for multiplate experiments.
Compatible with most plate types and slides.
Objectives
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Objective |
Image Resolution |
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4x Air |
1.699 µm / px |
|
10x Air |
679 nm / px |
|
20x Water |
338.7 nm / px |
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40x Water |
169.4 nm / px |
Filters equipped
Long term real-time, non-invasive, quantitative live-cell analysis; 4x, 10x, and 20x objectives, HD phase-contrast, widefield fluorescence.
The machine is in a 37C 5%CO2 incubator.
Live-cell monitoring of e.g. proliferation, cell death, wound-healing, scratch assays.
Compatible with most plate types.
Objectives
|
Objective |
Image Resolution |
|
4x Air |
2.82 µm / px |
|
10x Air |
1.24 µm / px |
|
20x Air |
0.62 µm / px |
Filters equipped
|
Filter set |
Excitation |
Emission |
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Green |
441-481 nm |
503-544 nm |
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Red |
567-607 nm |
622-704 nm |
Visit IncuCyte S3 Live-Cell Analysis System's website by clicking here.
High-throughput screening inverted widefield microscope from Olympus and designed for fully automated image acquisition and data analysis of biological samples. Available both with and without incubation chamber for live imaging with controlled CO2 and temperature.
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Fluorescence light source |
High power LED illumination system: Lumencor SpectraX light engine |
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Objectives |
UPlaFl 10x/ 0.3 AIR, UPlanSApo 20x / 0.75 AIR UPlanSApo 40x / 0.95 AIR. |
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Filters for fluorescence |
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Transmitted light techniques |
Brightfield |
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Camera |
Hamamatsu ORCA-FLASH 4.0, high-sensitivity digital monochrome scientific cooled sCMOS camera |
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Software |
ScanR Acquisition and Analysis V2.8 |
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format |
Well plate and slides |
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Location |
4.3.12 |
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Reference |
scanR High-Content Screening Station | Evident Scientific | Olympus |
Fully automated, inverted, multi-channel fluorescence and transmitted light imaging system coupled with capabilities for multi-position well scanning, Z-stack, tile-stitching, live-cell imaging, time lapse, and autofocus.
| Fluorescence light source | LED light sources |
| Objectives | 4X (AMEP4680), 10X (AMEP4681), 20X (AMEP4982), 40X (AMEP4683 and AMEP4754) |
| Filters for fluorescence |
GFP Ex 482/25, Em 524/24 RFP Ex 542/20, Em 593/40 |
| Transmitted light techniques | Brightfield |
| Camera | Colour and Monochrome camera |
| Software | Auto 2 |
| Format | Wellplate and slides |
| Location | 3.4.10 |
| Reference | The EVOS FL Auto Imaging System | Thermo Fisher Scientific - DK |
Inverted decovolution fluorescence widefield microscope with XYZ motorized stage/focus control, environmental control for live cell imaging, time lapse, multi point, and tile scanning from Olympus.
Compatible with most plate types and slides.
| Fluorescence light source | High power LED illumination system: Lumencor SpectraX light engine (https://lumencor.com/products/spectra-x-light-engine/) | ||||||||||||||||||||||||||||
| Objectives | UPlanSApo 10x/ 0.4 AIR, LCPlanFl 20x / 0.4 AIR, UApo/340 40x / 1.35 Oil, PlanApoN 60x/ 1.42 OIL, UPlanSApo 100x/ 1.4 OIL. | ||||||||||||||||||||||||||||
| Filters for fluorescence |
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| Transmitted light techniques | Brightfield and DIC | ||||||||||||||||||||||||||||
| Camera | Photometrics CoolSNAP HQ camera | ||||||||||||||||||||||||||||
| Software | SoftWoRx V 7.2.2 | ||||||||||||||||||||||||||||
| Format | 4.3.12 | ||||||||||||||||||||||||||||
| Reference | https://www.imsol.co.uk/deltavision-elite/ | ||||||||||||||||||||||||||||
Zeiss Laser Confocal Scanning Microscope with super-resolution Airyscan. Incubation chamber for live imaging with controlled CO2 and temperature. Microscope is equipped with four solid state lasers, a GaAsP PMT detectors and one T-PMT detector for transmission light detection.
Compatible with most plate types and slides.
|
Lasers |
405, 488, 561, 640 nm |
|---|---|
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Detectors |
1 x GaAsP PMT, 1 x T- PMT detector |
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Objectives |
Plan-Apochromat 10x / 0.45 AIR, Plan-Apochromat 25x / 0.80 IMM Plan-Apochromat 40x / 1.3 OIL, C-Apochromat 40x / 1.2 W, Plan-Apochromat 63x / 1.4 OIL. |
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Fluorescence light source |
HXP 120V |
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Filters for fluorescence |
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Transmitted light techniques |
Brightfield |
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Software |
ZEN Blue |
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Location |
3.3.14 |
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Reference |
Inverted microscope Leica SP8 is an automated inverted confocal laser scanning microscope with a very flexible white light laser source and super-sensitive Hybrid detectors (HyD)
Compatible with most plate types and slides.
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Lasers |
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Detectors |
2 x super-sensitive Hybrid detectors (HyD), 1 x T- PMT detector |
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Objectives |
HC Plan-Apochromat 10x / 0.40 AIR, HC Plan-Apochromat 20x / 0.75 IMM, HC Plan-Apochromat 40x / 1.1 W, HC Plan-Apochromat 63x / 1.40 OIL. |
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Fluorescence light source |
HXP 120V |
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Filters for fluorescence |
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Transmitted light techniques |
Brightfield |
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Software |
LASX |
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Location |
4.3.26 |
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Reference |
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Widefield upright microscope Zeiss AxioImager M2
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Fluorescence light source |
HXP 120V |
|---|---|
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Filters for fluorescence |
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Cameras |
Hamamatsu ORCA R2 |
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Objectives |
ECPlan-Neofluar 10x / 0.30 AIR, ECPlan-Neofluar 20x / 0.5 AIR, ECPlan-Neofluar 40x / 0.75 AIR, Plan-Apochromat 63x / 1.4 OIL, Plan-Apochromat 100x / 1.40 OIL |
|
Transmitted light techniques |
Brightfield, Differential interference contrast, Phase contrast, Darkfield |
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Software |
ZEN Blue |
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Location |
3.4.44 |
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Reference |
Plate readers
A plate reader with absorbance, fluorescence and luminescence capabilities, combined with brightfield and fluorescence well-imaging with single cell resolution.
Broad range of absorbance or luminescent based readouts. Nucleic acid concentration measurements.
Compatible with most plate types all readings completed at room temperature.
Objectives
|
Objective |
Resolution |
|
4x Air |
3.0 µm |
Filters equipped (for microscopy)
|
Filter set |
Excitation |
|
Blue |
385 nm LED |
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Green |
465 nm LED |
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Orange |
525 nm LED |
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Red |
632 nm LED |
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Gray |
735 nm LED or Transmitted Light |
Capabilities
Df
Applications
Ad
Objectives
F
Filters equipped (for microscopy)
Ad
Add a link to the machine
Automated Liquid Handling
Classic liquid handler with a 384 well head and 2 individual tips. Works at room temperature with heating and cooling modules for plates and reagents.
Works with volumes starting at 5µL in 0.1µL increments.
Mostly 96 and 384 well plates, other plate types or tubes might be compatible, contact LASM for more details.
Examples of use are automated cell splitting, 2D and 3D culture fixation and staining, high-throughput screening.
Acoustic liquid transfer, allowing for tip-free low volume transfer of liquid. All transfers take place at room temperature.
Echo550 for all liquid classes 2.5nL droplet size.
Echo525 for non-DMSO liquids 25nL droplet size.
Compatible with all standard plate types.
Examples of use are compound screening, dose response curves, antibody staining, miniaturized combinatorial reagent addition, low volume PCR reactions.
Peristaltic liquid dispensing in 8 channel or single channel format into 96 or 384 well plates.
Standard cassettes dispense in increments of 5µL, however 1µ and 10µL are also possible upon request.
Can be used at room temperature or 4C, on the bench or in a sterile hood.
Compatible with 96 and 384 well plates.
Excellent for cell seeding and general reagent dispensing.
Diaphragm based dispensing into any plate format with extremely low dead volume.
Dispenses liquid in 100nL increments from 100nL-5mL.
Can be used at room temperature, on the bench or in a sterile hood.
Compatible with all plate types.
Excellent for addition of a variety of agents to plates where the Echo might not be appropriate, or for situations where the dead volume is a factor.
Flow Cytometry
High throughput Flow cytometer designed to run standard 96 or 384-well plates. Key advantage is there is Zero dead volume required, the complete sample will be acquired.
Offering Violet (6 filters), Blue (5 filters) and Red (2 filters) laser lines.
Compatible with 96 and 384 well plates with 1µL - 90µL / well.
Used for drug discovery/development, screening assays, biomarker discovery/development and characterization of complex cellular populations.
Visit iQue Advanced Flow Cytometry´s website by clicking here.
Multiplexing Spatial Microscopy
Multiplexing inverted widefield microscope from Miltenyi Biotec and designed for fully automated cyclic staining and imaging of 50+ markers on a single sample or multiple samples, and data analysis of biological samples.
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Fluorescence light source |
LED illumination system |
|---|---|
|
Objectives |
2× objective to generate overview images; NA 0.1 20× long working distance objective (designed for 1 mm thick slides); NA 0.45 20× objective with high numerical aperture (designed for 170 μm thick cover glass); NA 0.75 |
|
Excitation |
Six excitation LEDs (filters: 386/23 nm, 420/10 nm, 470/40 nm, 531/46 nm, 628/32 nm, 725/40 nm) |
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Emission |
Five emission filters (470/40 nm, 530/43 nm, 580/25 nm, 698/70 nm, 809/81 nm) |
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Camera |
sCMOS camera (15 megapixel, 25 mm diagonal field of view) |
|
MACS Software |
MACSima™ (Acquisition); MACS iQ View (analysis) |
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Liquid handling system
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Robotic needle arm allowing fully automated liquid transfer
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MACS staining method |
Cyclic Staining (DAPI/FITC/PE/APC) |
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Autofocus |
Dual approach of hardware- and image-based autofocus mechanisms |
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Bleaching unit |
Illuminated area: 3 mm × 3 mm Light intensity: 2 W |
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Location |
3.4.50A |
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Format |
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Reference |
Spatial biology | MACSima™ Platform | Multiplex Imaging | Miltenyi Biotec | Deutschland |
Automation Platform
Biosero’s GBG is used to link a variety of the machines to complete larger plate number tasks. This can be simple dispensing, staining, or various readouts. A PF400 arm is used to move the plates to the various stations.
The machines which are on the platform can be used with or without the arm. The following are located on the platform:
- Echo550
- Echo525
- iQue 3
- ImageXpress Confocal
- Cytomat C2 incubator (37C, 5%CO2)
- BioTek MultiFlowFX (slightly different configuration than the stand-alone version)
- BioTek 405LS plate washer
- BioShake plate shaker
- BioNex HiG4 plate centrifuge
- Plate hotels for up to 45 plates in a single run
Pricing is determined by which machines are required for the run.
The LASM facility now has an environmentally controlled compound/drug storage system.
We currently host approximately 500 drugs and drug candidate molecules with defined mechanism of action which can be used both in screens and in bench-scale experiments.
Users can:
- Request aliquots of compounds for bench-scale testing
- Request the creation of ready to use drug plates
- Store their valuable compounds in a controlled atmosphere pod
Contact LASM (lasm@bric.ku.dk) to get a current list of compounds, inquire about specific compounds or compound targets, and pricing for your needs.
We offer arrayed CRISPR screening services for both whole‑genome and cherry‑picked gene sets. Thus, we can enable knockout or activation studies tailored to your biological question.
Our team supports the full process, from transfection optimization and assay development to data acquisition and analysis.
View the details on our available CRISPR libraries below and contact us to discuss your project:
Contact
LASM Lab
lasm@bric.ku.dk
BRIC - Biotech Research & Innovation
Centre Ole Maaløes Vej 5
DK-2200 Copenhagen
Room 4-4-32 (LASM Lab) or
3.4.37 (LASM Office).
Professor, Scientific Advisor
krister.wennerberg@bric.ku.dk
Bichitra Paul
Senior Core Facility Scientist
Assay Development and
CRISPR screening
bichitra.paul@bric.ku.dk
André Eduardo Carneiro Dias
Lead Core Facility Scientist
Imaging & Image analysis
andre.dias@bric.ku.dk
Sandra Gordon
Lead Core Facility Scientist
Automation & Screening
sandra.gordon@bric.ku.dk
Yasuko Antoku
Senior Core Facility Scientist
Imaging & Image analysis
Yasuko.Antoku@bric.ku.dk