In Vitro Silencing of lncRNA Expression Using siRNAs

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

In Vitro Silencing of lncRNA Expression Using siRNAs. / Thijssen, Meike S; Bintz, Jennifer; Arnes, Luis.

Long Non-Coding RNAs in Cancer. ed. / Alfons Navarro. Vol. 2348 Humana Press, 2021. p. 141-156 (Methods in molecular biology (Clifton, N.J.)).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Thijssen, MS, Bintz, J & Arnes, L 2021, In Vitro Silencing of lncRNA Expression Using siRNAs. in A Navarro (ed.), Long Non-Coding RNAs in Cancer. vol. 2348, Humana Press, Methods in molecular biology (Clifton, N.J.), pp. 141-156. https://doi.org/10.1007/978-1-0716-1581-2_9

APA

Thijssen, M. S., Bintz, J., & Arnes, L. (2021). In Vitro Silencing of lncRNA Expression Using siRNAs. In A. Navarro (Ed.), Long Non-Coding RNAs in Cancer (Vol. 2348, pp. 141-156). Humana Press. Methods in molecular biology (Clifton, N.J.) https://doi.org/10.1007/978-1-0716-1581-2_9

Vancouver

Thijssen MS, Bintz J, Arnes L. In Vitro Silencing of lncRNA Expression Using siRNAs. In Navarro A, editor, Long Non-Coding RNAs in Cancer. Vol. 2348. Humana Press. 2021. p. 141-156. (Methods in molecular biology (Clifton, N.J.)). https://doi.org/10.1007/978-1-0716-1581-2_9

Author

Thijssen, Meike S ; Bintz, Jennifer ; Arnes, Luis. / In Vitro Silencing of lncRNA Expression Using siRNAs. Long Non-Coding RNAs in Cancer. editor / Alfons Navarro. Vol. 2348 Humana Press, 2021. pp. 141-156 (Methods in molecular biology (Clifton, N.J.)).

Bibtex

@inbook{3609780c4efb45df93ab7b812edb9908,
title = "In Vitro Silencing of lncRNA Expression Using siRNAs",
abstract = "Recent advances in sequencing technologies have uncovered the existence of thousands of long noncoding RNAs (lncRNAs) with dysregulated expression in cancer. As a result, there is burgeoning interest in understanding their function and biological significance in both homeostasis and disease. RNA interference (RNAi) enables sequence-specific gene silencing and can, in principle, be employed to silence virtually any gene. However, when applied to lncRNAs, it is important to consider current limitations in their annotation and current principles regarding lncRNA regulation and function when assessing their phenotype in cancer cell lines. In this chapter we describe the analysis of lncRNA splicing variant expression, including subcellular localization, transfection of siRNAs in cancer cell lines, and validation of gene silencing by quantitative PCR and single molecule in situ hybridization. All protocols can be performed in a laboratory with essential equipment for cell culture, molecular biology, and imaging.",
author = "Thijssen, {Meike S} and Jennifer Bintz and Luis Arnes",
year = "2021",
doi = "10.1007/978-1-0716-1581-2_9",
language = "English",
volume = "2348",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Humana Press",
pages = "141--156",
editor = "Alfons Navarro",
booktitle = "Long Non-Coding RNAs in Cancer",
address = "United States",

}

RIS

TY - CHAP

T1 - In Vitro Silencing of lncRNA Expression Using siRNAs

AU - Thijssen, Meike S

AU - Bintz, Jennifer

AU - Arnes, Luis

PY - 2021

Y1 - 2021

N2 - Recent advances in sequencing technologies have uncovered the existence of thousands of long noncoding RNAs (lncRNAs) with dysregulated expression in cancer. As a result, there is burgeoning interest in understanding their function and biological significance in both homeostasis and disease. RNA interference (RNAi) enables sequence-specific gene silencing and can, in principle, be employed to silence virtually any gene. However, when applied to lncRNAs, it is important to consider current limitations in their annotation and current principles regarding lncRNA regulation and function when assessing their phenotype in cancer cell lines. In this chapter we describe the analysis of lncRNA splicing variant expression, including subcellular localization, transfection of siRNAs in cancer cell lines, and validation of gene silencing by quantitative PCR and single molecule in situ hybridization. All protocols can be performed in a laboratory with essential equipment for cell culture, molecular biology, and imaging.

AB - Recent advances in sequencing technologies have uncovered the existence of thousands of long noncoding RNAs (lncRNAs) with dysregulated expression in cancer. As a result, there is burgeoning interest in understanding their function and biological significance in both homeostasis and disease. RNA interference (RNAi) enables sequence-specific gene silencing and can, in principle, be employed to silence virtually any gene. However, when applied to lncRNAs, it is important to consider current limitations in their annotation and current principles regarding lncRNA regulation and function when assessing their phenotype in cancer cell lines. In this chapter we describe the analysis of lncRNA splicing variant expression, including subcellular localization, transfection of siRNAs in cancer cell lines, and validation of gene silencing by quantitative PCR and single molecule in situ hybridization. All protocols can be performed in a laboratory with essential equipment for cell culture, molecular biology, and imaging.

U2 - 10.1007/978-1-0716-1581-2_9

DO - 10.1007/978-1-0716-1581-2_9

M3 - Book chapter

C2 - 34160804

VL - 2348

T3 - Methods in molecular biology (Clifton, N.J.)

SP - 141

EP - 156

BT - Long Non-Coding RNAs in Cancer

A2 - Navarro, Alfons

PB - Humana Press

ER -

ID: 275776962