Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment. / Lauridsen, Felicia KB.; Jensen, Tanja L.; Rapin, Nicolas; Aslan, Derya; Wilhelmson, Anna S.; Pundhir, Sachin; Rehn, Matilda; Paul, Franziska; Giladi, Amir; Hasemann, Marie Sigurd; Serup, Palle; Amit, Ido; Porse, Bo Torben.

In: Cell Reports, Vol. 24, No. 3, 2018, p. 766-780.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lauridsen, FKB, Jensen, TL, Rapin, N, Aslan, D, Wilhelmson, AS, Pundhir, S, Rehn, M, Paul, F, Giladi, A, Hasemann, MS, Serup, P, Amit, I & Porse, BT 2018, 'Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment', Cell Reports, vol. 24, no. 3, pp. 766-780. https://doi.org/10.1016/j.celrep.2018.06.057

APA

Lauridsen, F. KB., Jensen, T. L., Rapin, N., Aslan, D., Wilhelmson, A. S., Pundhir, S., Rehn, M., Paul, F., Giladi, A., Hasemann, M. S., Serup, P., Amit, I., & Porse, B. T. (2018). Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment. Cell Reports, 24(3), 766-780. https://doi.org/10.1016/j.celrep.2018.06.057

Vancouver

Lauridsen FKB, Jensen TL, Rapin N, Aslan D, Wilhelmson AS, Pundhir S et al. Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment. Cell Reports. 2018;24(3):766-780. https://doi.org/10.1016/j.celrep.2018.06.057

Author

Lauridsen, Felicia KB. ; Jensen, Tanja L. ; Rapin, Nicolas ; Aslan, Derya ; Wilhelmson, Anna S. ; Pundhir, Sachin ; Rehn, Matilda ; Paul, Franziska ; Giladi, Amir ; Hasemann, Marie Sigurd ; Serup, Palle ; Amit, Ido ; Porse, Bo Torben. / Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment. In: Cell Reports. 2018 ; Vol. 24, No. 3. pp. 766-780.

Bibtex

@article{75679727b0e44225856b341ee8a606a5,

title = "Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment",

abstract = "Hematopoietic stem cells (HSCs) are considered a heterogeneous cell population. To further resolve the HSC compartment, we characterized a retinoic acid (RA) reporter mouse line. Sub-fractionation of the HSC compartment in RA-CFP reporter mice demonstrated that RA-CFP-dim HSCs were largely non-proliferative and displayed superior engraftment potential in comparison with RA-CFP-bright HSCs. Gene expression analysis demonstrated higher expression of RA-target genes in RA-CFP-dim HSCs, in contrast to the RA-CFP reporter expression, but both RA-CFP-dim and RA-CFP-bright HSCs responded efficiently to RA in vitro. Single-cell RNA sequencing (RNA-seq) of >1,200 HSCs showed that differences in cell cycle activity constituted the main driver of transcriptional heterogeneity in HSCs. Moreover, further analysis of the single-cell RNA-seq data revealed that stochastic low-level expression of distinct lineage-affiliated transcriptional programs is a common feature of HSCs. Collectively, this work demonstrates the utility of the RA-CFP reporter line as a tool for the isolation of superior HSCs.",

author = "Lauridsen, {Felicia KB.} and Jensen, {Tanja L.} and Nicolas Rapin and Derya Aslan and Wilhelmson, {Anna S.} and Sachin Pundhir and Matilda Rehn and Franziska Paul and Amir Giladi and Hasemann, {Marie Sigurd} and Palle Serup and Ido Amit and Porse, {Bo Torben}",

year = "2018",

doi = "10.1016/j.celrep.2018.06.057",

language = "English",

volume = "24",

pages = "766--780",

journal = "Cell Reports",

issn = "2211-1247",

publisher = "Cell Press",

number = "3",

}

RIS

TY - JOUR

T1 - Differences in Cell Cycle Status Underlie Transcriptional Heterogeneity in the HSC Compartment

AU - Lauridsen, Felicia KB.

AU - Jensen, Tanja L.

AU - Rapin, Nicolas

AU - Aslan, Derya

AU - Wilhelmson, Anna S.

AU - Pundhir, Sachin

AU - Rehn, Matilda

AU - Paul, Franziska

AU - Giladi, Amir

AU - Hasemann, Marie Sigurd

AU - Serup, Palle

AU - Amit, Ido

AU - Porse, Bo Torben

PY - 2018

Y1 - 2018

N2 - Hematopoietic stem cells (HSCs) are considered a heterogeneous cell population. To further resolve the HSC compartment, we characterized a retinoic acid (RA) reporter mouse line. Sub-fractionation of the HSC compartment in RA-CFP reporter mice demonstrated that RA-CFP-dim HSCs were largely non-proliferative and displayed superior engraftment potential in comparison with RA-CFP-bright HSCs. Gene expression analysis demonstrated higher expression of RA-target genes in RA-CFP-dim HSCs, in contrast to the RA-CFP reporter expression, but both RA-CFP-dim and RA-CFP-bright HSCs responded efficiently to RA in vitro. Single-cell RNA sequencing (RNA-seq) of >1,200 HSCs showed that differences in cell cycle activity constituted the main driver of transcriptional heterogeneity in HSCs. Moreover, further analysis of the single-cell RNA-seq data revealed that stochastic low-level expression of distinct lineage-affiliated transcriptional programs is a common feature of HSCs. Collectively, this work demonstrates the utility of the RA-CFP reporter line as a tool for the isolation of superior HSCs.

AB - Hematopoietic stem cells (HSCs) are considered a heterogeneous cell population. To further resolve the HSC compartment, we characterized a retinoic acid (RA) reporter mouse line. Sub-fractionation of the HSC compartment in RA-CFP reporter mice demonstrated that RA-CFP-dim HSCs were largely non-proliferative and displayed superior engraftment potential in comparison with RA-CFP-bright HSCs. Gene expression analysis demonstrated higher expression of RA-target genes in RA-CFP-dim HSCs, in contrast to the RA-CFP reporter expression, but both RA-CFP-dim and RA-CFP-bright HSCs responded efficiently to RA in vitro. Single-cell RNA sequencing (RNA-seq) of >1,200 HSCs showed that differences in cell cycle activity constituted the main driver of transcriptional heterogeneity in HSCs. Moreover, further analysis of the single-cell RNA-seq data revealed that stochastic low-level expression of distinct lineage-affiliated transcriptional programs is a common feature of HSCs. Collectively, this work demonstrates the utility of the RA-CFP reporter line as a tool for the isolation of superior HSCs.

U2 - 10.1016/j.celrep.2018.06.057

DO - 10.1016/j.celrep.2018.06.057

M3 - Journal article

C2 - 30021172

VL - 24

SP - 766

EP - 780

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 3

ER -

ID: 200815524

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