Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression

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Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression. / Rafaeva, Maria; Jensen, Adina R.D.; Horton, Edward R.; Zornhagen, Kamilla W.; Strøbech, Jan E.; Fleischhauer, Lutz; Mayorca-Guiliani, Alejandro E.; Nielsen, Sebastian R.; Grønseth, Dina S.; Kuś, Filip; Schoof, Erwin M.; Arnes, Luis; Koch, Manuel; Clausen-Schaumann, Hauke; Izzi, Valerio; Reuten, Raphael; Erler, Janine T.

In: Frontiers in Immunology, Vol. 14, 1154528, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rafaeva, M, Jensen, ARD, Horton, ER, Zornhagen, KW, Strøbech, JE, Fleischhauer, L, Mayorca-Guiliani, AE, Nielsen, SR, Grønseth, DS, Kuś, F, Schoof, EM, Arnes, L, Koch, M, Clausen-Schaumann, H, Izzi, V, Reuten, R & Erler, JT 2023, 'Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression', Frontiers in Immunology, vol. 14, 1154528. https://doi.org/10.3389/fimmu.2023.1154528

APA

Rafaeva, M., Jensen, A. R. D., Horton, E. R., Zornhagen, K. W., Strøbech, J. E., Fleischhauer, L., Mayorca-Guiliani, A. E., Nielsen, S. R., Grønseth, D. S., Kuś, F., Schoof, E. M., Arnes, L., Koch, M., Clausen-Schaumann, H., Izzi, V., Reuten, R., & Erler, J. T. (2023). Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression. Frontiers in Immunology, 14, [1154528]. https://doi.org/10.3389/fimmu.2023.1154528

Vancouver

Rafaeva M, Jensen ARD, Horton ER, Zornhagen KW, Strøbech JE, Fleischhauer L et al. Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression. Frontiers in Immunology. 2023;14. 1154528. https://doi.org/10.3389/fimmu.2023.1154528

Author

Rafaeva, Maria ; Jensen, Adina R.D. ; Horton, Edward R. ; Zornhagen, Kamilla W. ; Strøbech, Jan E. ; Fleischhauer, Lutz ; Mayorca-Guiliani, Alejandro E. ; Nielsen, Sebastian R. ; Grønseth, Dina S. ; Kuś, Filip ; Schoof, Erwin M. ; Arnes, Luis ; Koch, Manuel ; Clausen-Schaumann, Hauke ; Izzi, Valerio ; Reuten, Raphael ; Erler, Janine T. / Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression. In: Frontiers in Immunology. 2023 ; Vol. 14.

Bibtex

@article{473ba659347d48369fd0c89b8f225769,
title = "Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression",
abstract = "The desmoplastic reaction observed in many cancers is a hallmark of disease progression and prognosis, particularly in breast and pancreatic cancer. Stromal-derived extracellular matrix (ECM) is significantly altered in desmoplasia, and as such plays a critical role in driving cancer progression. Using fibroblast-derived matrices (FDMs), we show that cancer cells have increased growth on cancer associated FDMs, when compared to FDMs derived from non-malignant tissue (normal) fibroblasts. We assess the changes in ECM characteristics from normal to cancer-associated stroma at the primary tumor site. Compositional, structural, and mechanical analyses reveal significant differences, with an increase in abundance of core ECM proteins, coupled with an increase in stiffness and density in cancer-associated FDMs. From compositional changes of FDM, we derived a 36-ECM protein signature, which we show matches in large part with the changes in pancreatic ductal adenocarcinoma (PDAC) tumor and metastases progression. Additionally, this signature also matches at the transcriptomic level in multiple cancer types in patients, prognostic of their survival. Together, our results show relevance of FDMs for cancer modelling and identification of desmoplastic ECM components for further mechanistic studies.",
keywords = "breast cancer, desmoplasia, extracellular matrix, fibroblasts, mechanics, models, pancreatic cancer",
author = "Maria Rafaeva and Jensen, {Adina R.D.} and Horton, {Edward R.} and Zornhagen, {Kamilla W.} and Str{\o}bech, {Jan E.} and Lutz Fleischhauer and Mayorca-Guiliani, {Alejandro E.} and Nielsen, {Sebastian R.} and Gr{\o}nseth, {Dina S.} and Filip Ku{\'s} and Schoof, {Erwin M.} and Luis Arnes and Manuel Koch and Hauke Clausen-Schaumann and Valerio Izzi and Raphael Reuten and Erler, {Janine T.}",
note = "Publisher Copyright: Copyright {\textcopyright} 2023 Rafaeva, Jensen, Horton, Zornhagen, Str{\o}bech, Fleischhauer, Mayorca-Guiliani, Nielsen, Gr{\o}nseth, Ku{\'s}, Schoof, Arnes, Koch, Clausen-Schaumann, Izzi, Reuten and Erler.",
year = "2023",
doi = "10.3389/fimmu.2023.1154528",
language = "English",
volume = "14",
journal = "Frontiers in Immunology",
issn = "1664-3224",
publisher = "Frontiers Research Foundation",

}

RIS

TY - JOUR

T1 - Fibroblast-derived matrix models desmoplastic properties and forms a prognostic signature in cancer progression

AU - Rafaeva, Maria

AU - Jensen, Adina R.D.

AU - Horton, Edward R.

AU - Zornhagen, Kamilla W.

AU - Strøbech, Jan E.

AU - Fleischhauer, Lutz

AU - Mayorca-Guiliani, Alejandro E.

AU - Nielsen, Sebastian R.

AU - Grønseth, Dina S.

AU - Kuś, Filip

AU - Schoof, Erwin M.

AU - Arnes, Luis

AU - Koch, Manuel

AU - Clausen-Schaumann, Hauke

AU - Izzi, Valerio

AU - Reuten, Raphael

AU - Erler, Janine T.

N1 - Publisher Copyright: Copyright © 2023 Rafaeva, Jensen, Horton, Zornhagen, Strøbech, Fleischhauer, Mayorca-Guiliani, Nielsen, Grønseth, Kuś, Schoof, Arnes, Koch, Clausen-Schaumann, Izzi, Reuten and Erler.

PY - 2023

Y1 - 2023

N2 - The desmoplastic reaction observed in many cancers is a hallmark of disease progression and prognosis, particularly in breast and pancreatic cancer. Stromal-derived extracellular matrix (ECM) is significantly altered in desmoplasia, and as such plays a critical role in driving cancer progression. Using fibroblast-derived matrices (FDMs), we show that cancer cells have increased growth on cancer associated FDMs, when compared to FDMs derived from non-malignant tissue (normal) fibroblasts. We assess the changes in ECM characteristics from normal to cancer-associated stroma at the primary tumor site. Compositional, structural, and mechanical analyses reveal significant differences, with an increase in abundance of core ECM proteins, coupled with an increase in stiffness and density in cancer-associated FDMs. From compositional changes of FDM, we derived a 36-ECM protein signature, which we show matches in large part with the changes in pancreatic ductal adenocarcinoma (PDAC) tumor and metastases progression. Additionally, this signature also matches at the transcriptomic level in multiple cancer types in patients, prognostic of their survival. Together, our results show relevance of FDMs for cancer modelling and identification of desmoplastic ECM components for further mechanistic studies.

AB - The desmoplastic reaction observed in many cancers is a hallmark of disease progression and prognosis, particularly in breast and pancreatic cancer. Stromal-derived extracellular matrix (ECM) is significantly altered in desmoplasia, and as such plays a critical role in driving cancer progression. Using fibroblast-derived matrices (FDMs), we show that cancer cells have increased growth on cancer associated FDMs, when compared to FDMs derived from non-malignant tissue (normal) fibroblasts. We assess the changes in ECM characteristics from normal to cancer-associated stroma at the primary tumor site. Compositional, structural, and mechanical analyses reveal significant differences, with an increase in abundance of core ECM proteins, coupled with an increase in stiffness and density in cancer-associated FDMs. From compositional changes of FDM, we derived a 36-ECM protein signature, which we show matches in large part with the changes in pancreatic ductal adenocarcinoma (PDAC) tumor and metastases progression. Additionally, this signature also matches at the transcriptomic level in multiple cancer types in patients, prognostic of their survival. Together, our results show relevance of FDMs for cancer modelling and identification of desmoplastic ECM components for further mechanistic studies.

KW - breast cancer

KW - desmoplasia

KW - extracellular matrix

KW - fibroblasts

KW - mechanics

KW - models

KW - pancreatic cancer

U2 - 10.3389/fimmu.2023.1154528

DO - 10.3389/fimmu.2023.1154528

M3 - Journal article

C2 - 37539058

AN - SCOPUS:85166431556

VL - 14

JO - Frontiers in Immunology

JF - Frontiers in Immunology

SN - 1664-3224

M1 - 1154528

ER -

ID: 362297073