Regulation of translation by site-specific ribosomal RNA methylation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Regulation of translation by site-specific ribosomal RNA methylation. / Jansson, Martin David; Häfner, Sophia Julia; Altinel, Kübra; Tehler, Disa Elisabet; Krogh, Nicolai; Jakobsen, Emil; Andersen, Jens Velde; Andersen, Kasper Langebjerg; Schoof, Erwin; Ménard, Patrice; Nielsen, Henrik; Lund, Anders H.

In: Nature Structural and Molecular Biology, Vol. 28, No. 11, 2021, p. 889-899.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jansson, MD, Häfner, SJ, Altinel, K, Tehler, DE, Krogh, N, Jakobsen, E, Andersen, JV, Andersen, KL, Schoof, E, Ménard, P, Nielsen, H & Lund, AH 2021, 'Regulation of translation by site-specific ribosomal RNA methylation', Nature Structural and Molecular Biology, vol. 28, no. 11, pp. 889-899. https://doi.org/10.1038/s41594-021-00669-4

APA

Jansson, M. D., Häfner, S. J., Altinel, K., Tehler, D. E., Krogh, N., Jakobsen, E., Andersen, J. V., Andersen, K. L., Schoof, E., Ménard, P., Nielsen, H., & Lund, A. H. (2021). Regulation of translation by site-specific ribosomal RNA methylation. Nature Structural and Molecular Biology, 28(11), 889-899. https://doi.org/10.1038/s41594-021-00669-4

Vancouver

Jansson MD, Häfner SJ, Altinel K, Tehler DE, Krogh N, Jakobsen E et al. Regulation of translation by site-specific ribosomal RNA methylation. Nature Structural and Molecular Biology. 2021;28(11):889-899. https://doi.org/10.1038/s41594-021-00669-4

Author

Jansson, Martin David ; Häfner, Sophia Julia ; Altinel, Kübra ; Tehler, Disa Elisabet ; Krogh, Nicolai ; Jakobsen, Emil ; Andersen, Jens Velde ; Andersen, Kasper Langebjerg ; Schoof, Erwin ; Ménard, Patrice ; Nielsen, Henrik ; Lund, Anders H. / Regulation of translation by site-specific ribosomal RNA methylation. In: Nature Structural and Molecular Biology. 2021 ; Vol. 28, No. 11. pp. 889-899.

Bibtex

@article{8382c2ce22f244b582ca7f76d1f820f6,
title = "Regulation of translation by site-specific ribosomal RNA methylation",
abstract = "Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented in several organisms and can arise from several different sources. A key question is whether specific control over ribosome heterogeneity represents a mechanism by which translation can be regulated. We used RiboMeth-seq to demonstrate that differential 2′-O-methylation of ribosomal RNA (rRNA) represents a considerable source of ribosome heterogeneity in human cells, and that modification levels at distinct sites can change dynamically in response to upstream signaling pathways, such as MYC oncogene expression. Ablation of one prominent methylation resulted in altered translation of select mRNAs and corresponding changes in cellular phenotypes. Thus, differential rRNA 2′-O-methylation can give rise to ribosomes with specialized function. This suggests a broader mechanism where the specific regulation of rRNA modification patterns fine tunes translation.",
author = "Jansson, {Martin David} and H{\"a}fner, {Sophia Julia} and K{\"u}bra Altinel and Tehler, {Disa Elisabet} and Nicolai Krogh and Emil Jakobsen and Andersen, {Jens Velde} and Andersen, {Kasper Langebjerg} and Erwin Schoof and Patrice M{\'e}nard and Henrik Nielsen and Lund, {Anders H.}",
year = "2021",
doi = "10.1038/s41594-021-00669-4",
language = "English",
volume = "28",
pages = "889--899",
journal = "Nature Structural and Molecular Biology",
issn = "1545-9993",
publisher = "nature publishing group",
number = "11",

}

RIS

TY - JOUR

T1 - Regulation of translation by site-specific ribosomal RNA methylation

AU - Jansson, Martin David

AU - Häfner, Sophia Julia

AU - Altinel, Kübra

AU - Tehler, Disa Elisabet

AU - Krogh, Nicolai

AU - Jakobsen, Emil

AU - Andersen, Jens Velde

AU - Andersen, Kasper Langebjerg

AU - Schoof, Erwin

AU - Ménard, Patrice

AU - Nielsen, Henrik

AU - Lund, Anders H.

PY - 2021

Y1 - 2021

N2 - Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented in several organisms and can arise from several different sources. A key question is whether specific control over ribosome heterogeneity represents a mechanism by which translation can be regulated. We used RiboMeth-seq to demonstrate that differential 2′-O-methylation of ribosomal RNA (rRNA) represents a considerable source of ribosome heterogeneity in human cells, and that modification levels at distinct sites can change dynamically in response to upstream signaling pathways, such as MYC oncogene expression. Ablation of one prominent methylation resulted in altered translation of select mRNAs and corresponding changes in cellular phenotypes. Thus, differential rRNA 2′-O-methylation can give rise to ribosomes with specialized function. This suggests a broader mechanism where the specific regulation of rRNA modification patterns fine tunes translation.

AB - Ribosomes are complex ribozymes that interpret genetic information by translating messenger RNA (mRNA) into proteins. Natural variation in ribosome composition has been documented in several organisms and can arise from several different sources. A key question is whether specific control over ribosome heterogeneity represents a mechanism by which translation can be regulated. We used RiboMeth-seq to demonstrate that differential 2′-O-methylation of ribosomal RNA (rRNA) represents a considerable source of ribosome heterogeneity in human cells, and that modification levels at distinct sites can change dynamically in response to upstream signaling pathways, such as MYC oncogene expression. Ablation of one prominent methylation resulted in altered translation of select mRNAs and corresponding changes in cellular phenotypes. Thus, differential rRNA 2′-O-methylation can give rise to ribosomes with specialized function. This suggests a broader mechanism where the specific regulation of rRNA modification patterns fine tunes translation.

U2 - 10.1038/s41594-021-00669-4

DO - 10.1038/s41594-021-00669-4

M3 - Journal article

C2 - 34759377

VL - 28

SP - 889

EP - 899

JO - Nature Structural and Molecular Biology

JF - Nature Structural and Molecular Biology

SN - 1545-9993

IS - 11

ER -

ID: 284343865