GDNF and neublastin protect against NMDA-induced excitotoxicity in hippocampal slice cultures
Research output: Contribution to journal › Journal article › Research › peer-review
The potential neuroprotective effects of glial cell line-derived neurotrophic factor (GDNF) and neublastin (NBN) against NMDA-induced excitotoxicity were examined in hippocampal brain slice cultures. Recombinant human GDNF (25-100 ng/ ml) or NBN, in medium conditioned by growth of transfected, NBN-producing HiB5 cells, were added to slice cultures I h before exposure to 10 microM NMDA for 48h. Neuronal cell death was monitored, before and during the NMDA exposure, by densitometric measurements of propidium iodide (PI) uptake and loss of Nissl staining. Both the addition of rhGDNF and NBN-containing medium significantly reduced the NMDA-induced PI uptake in the CA1 (p < 0.01), suggesting neuroprotective effects of these factors, beyond their well-known trophic effects on dopaminergic neurons.
Original language | English |
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Journal | NeuroReport |
Volume | 11 |
Issue number | 18 |
Pages (from-to) | 4069-73 |
Number of pages | 5 |
ISSN | 0959-4965 |
DOIs | |
Publication status | Published - 18 Dec 2000 |
Externally published | Yes |
ID: 364508513