Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia
Research output: Contribution to journal › Journal article › Research › peer-review
AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.
MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.
RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.
CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.
| Original language | English |
|---|---|
| Journal | CNS oncology |
| Volume | 7 |
| Issue number | 2 |
| Pages (from-to) | CNS15 |
| ISSN | 2045-0907 |
| DOIs | |
| Publication status | Published - Apr 2018 |
| Externally published | Yes |
ID: 364506250