The GABAA receptor agonist THIP is neuroprotective in organotypic hippocampal slice cultures
Research output: Contribution to journal › Journal article › Research › peer-review
The potential neuroprotective effects of the GABA(A) receptor agonists THIP (4,5,6,7-tetrahydroisoxazolo[5,4-c]pyridin-3-ol) and muscimol, and the selective GluR5 kainate receptor agonist ATPA ((RS)-2-amino-3-(3-hydroxy-5-tert-butylisoxazol-4-yl)propanoic acid), which activates GABAergic interneurons, were examined in hippocampal slice cultures exposed to N-methyl-D-aspartate (NMDA). The NMDA-induced excitotoxicity was quantified by densitometric measurements of propidium iodide (PI) uptake. THIP (100-1000 microM) was neuroprotective in slice cultures co-exposed to NMDA (10 microM) for 48 h, while muscimol (100-1000 microM) and ATPA (1-3 microM) were without effect. The results demonstrate that direct GABA(A) agonism can mediate neuroprotection in the hippocampus in vitro as previously suggested in vivo.
Original language | English |
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Journal | Brain Research |
Volume | 973 |
Issue number | 2 |
Pages (from-to) | 303-6 |
Number of pages | 4 |
ISSN | 0006-8993 |
DOIs | |
Publication status | Published - 30 May 2003 |
Externally published | Yes |
ID: 364505238