Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR. / Bryson, James W.; Rosser, Susan J.

Mammalian Synthetic Systems. ed. / Francesca Ceroni; Karen Polizzi. Humana Press, 2024. p. 193-204 (Methods in Molecular Biology, Vol. 2774).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Bryson, JW & Rosser, SJ 2024, Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR. in F Ceroni & K Polizzi (eds), Mammalian Synthetic Systems. Humana Press, Methods in Molecular Biology, vol. 2774, pp. 193-204. https://doi.org/10.1007/978-1-0716-3718-0_13

APA

Bryson, J. W., & Rosser, S. J. (2024). Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR. In F. Ceroni, & K. Polizzi (Eds.), Mammalian Synthetic Systems (pp. 193-204). Humana Press. Methods in Molecular Biology Vol. 2774 https://doi.org/10.1007/978-1-0716-3718-0_13

Vancouver

Bryson JW, Rosser SJ. Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR. In Ceroni F, Polizzi K, editors, Mammalian Synthetic Systems. Humana Press. 2024. p. 193-204. (Methods in Molecular Biology, Vol. 2774). https://doi.org/10.1007/978-1-0716-3718-0_13

Author

Bryson, James W. ; Rosser, Susan J. / Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR. Mammalian Synthetic Systems. editor / Francesca Ceroni ; Karen Polizzi. Humana Press, 2024. pp. 193-204 (Methods in Molecular Biology, Vol. 2774).

Bibtex

@inbook{ccc70685d6c145819a2d01d73e2dd9cc,
title = "Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR",
abstract = "CRISPR activation provides an invaluable tool for experimental biologists to convert correlations into causation by directly observing phenotypic changes upon targeted changes in gene expression. With few exceptions, most diseases are caused by complex polygenic interactions, with multiple genes contributing to define the output of a gene network. As such researchers are increasingly interested in tools that can offer not only control but also the capacity to simultaneously upregulate multiple genes. The adaptation of CRISPR/Cas12a has provided a system especially suited to the tightly coordinated overexpression of multiple targeted genes. Here we describe an approach to test for active targeting crRNAs for dFnCas12a-VPR, before proceeding to generate and validate longer crRNA arrays for multiplexed targeting of genes of interest.",
keywords = "Cas12a, CRISPRa, crRNA assembly, Multiplexed activation",
author = "Bryson, {James W.} and Rosser, {Susan J.}",
note = "Publisher Copyright: {\textcopyright} 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
doi = "10.1007/978-1-0716-3718-0_13",
language = "English",
isbn = "978-1-0716-3717-3",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "193--204",
editor = "Francesca Ceroni and Karen Polizzi",
booktitle = "Mammalian Synthetic Systems",
address = "United States",

}

RIS

TY - CHAP

T1 - Multiplexed Transactivation of Mammalian Cells Using dFnCas12a-VPR

AU - Bryson, James W.

AU - Rosser, Susan J.

N1 - Publisher Copyright: © 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2024

Y1 - 2024

N2 - CRISPR activation provides an invaluable tool for experimental biologists to convert correlations into causation by directly observing phenotypic changes upon targeted changes in gene expression. With few exceptions, most diseases are caused by complex polygenic interactions, with multiple genes contributing to define the output of a gene network. As such researchers are increasingly interested in tools that can offer not only control but also the capacity to simultaneously upregulate multiple genes. The adaptation of CRISPR/Cas12a has provided a system especially suited to the tightly coordinated overexpression of multiple targeted genes. Here we describe an approach to test for active targeting crRNAs for dFnCas12a-VPR, before proceeding to generate and validate longer crRNA arrays for multiplexed targeting of genes of interest.

AB - CRISPR activation provides an invaluable tool for experimental biologists to convert correlations into causation by directly observing phenotypic changes upon targeted changes in gene expression. With few exceptions, most diseases are caused by complex polygenic interactions, with multiple genes contributing to define the output of a gene network. As such researchers are increasingly interested in tools that can offer not only control but also the capacity to simultaneously upregulate multiple genes. The adaptation of CRISPR/Cas12a has provided a system especially suited to the tightly coordinated overexpression of multiple targeted genes. Here we describe an approach to test for active targeting crRNAs for dFnCas12a-VPR, before proceeding to generate and validate longer crRNA arrays for multiplexed targeting of genes of interest.

KW - Cas12a

KW - CRISPRa

KW - crRNA assembly

KW - Multiplexed activation

U2 - 10.1007/978-1-0716-3718-0_13

DO - 10.1007/978-1-0716-3718-0_13

M3 - Book chapter

C2 - 38441766

AN - SCOPUS:85186846057

SN - 978-1-0716-3717-3

SN - 978-1-0716-3720-3

T3 - Methods in Molecular Biology

SP - 193

EP - 204

BT - Mammalian Synthetic Systems

A2 - Ceroni, Francesca

A2 - Polizzi, Karen

PB - Humana Press

ER -

ID: 385648462