Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay. / Kimura, Takao; Miyashita, Kazuya; Fukamachi, Isamu; Fukamachi, Kumiko; Ogura, Kazumi; Yokoyama, Erina; Tsunekawa, Katsuhiko; Nagasawa, Takumi; Ploug, Michael; Yang, Ye; Song, Wenxin; Young, Stephen G.; Beigneux, Anne P.; Nakajima, Katsuyuki; Murakami, Masami.

In: Journal of Lipid Research, Vol. 65, No. 4, 100532, 2024.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kimura, T, Miyashita, K, Fukamachi, I, Fukamachi, K, Ogura, K, Yokoyama, E, Tsunekawa, K, Nagasawa, T, Ploug, M, Yang, Y, Song, W, Young, SG, Beigneux, AP, Nakajima, K & Murakami, M 2024, 'Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay', Journal of Lipid Research, vol. 65, no. 4, 100532. https://doi.org/10.1016/j.jlr.2024.100532

APA

Kimura, T., Miyashita, K., Fukamachi, I., Fukamachi, K., Ogura, K., Yokoyama, E., Tsunekawa, K., Nagasawa, T., Ploug, M., Yang, Y., Song, W., Young, S. G., Beigneux, A. P., Nakajima, K., & Murakami, M. (2024). Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay. Journal of Lipid Research, 65(4), [100532]. https://doi.org/10.1016/j.jlr.2024.100532

Vancouver

Kimura T, Miyashita K, Fukamachi I, Fukamachi K, Ogura K, Yokoyama E et al. Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay. Journal of Lipid Research. 2024;65(4). 100532. https://doi.org/10.1016/j.jlr.2024.100532

Author

Kimura, Takao ; Miyashita, Kazuya ; Fukamachi, Isamu ; Fukamachi, Kumiko ; Ogura, Kazumi ; Yokoyama, Erina ; Tsunekawa, Katsuhiko ; Nagasawa, Takumi ; Ploug, Michael ; Yang, Ye ; Song, Wenxin ; Young, Stephen G. ; Beigneux, Anne P. ; Nakajima, Katsuyuki ; Murakami, Masami. / Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay. In: Journal of Lipid Research. 2024 ; Vol. 65, No. 4.

Bibtex

@article{e581490085444e0c9d5a02342195c570,

title = "Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay",

abstract = "To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrations ranging from 0.31 ng/ml to 20 ng/ ml. The sensitivity of the ELISA made it possible to quantify LPL in serum and in both pre-heparin and post-heparin plasma samples (including in grossly lipemic samples). LPL mass and activity levels in the post-heparin plasma were lower in Gpihbp1¡/¡ mice than in wild-type mice. In both groups of mice, LPL mass and activity levels were positively correlated. Our mAb-based sandwich ELISA for mouse LPL will be useful for any investigator who uses mouse models to study LPL-mediated intravascular lipolysis.",

keywords = "GPIHBP1, HTGL, lipids, LPL, transport, triglycerides, vascular biology",

author = "Takao Kimura and Kazuya Miyashita and Isamu Fukamachi and Kumiko Fukamachi and Kazumi Ogura and Erina Yokoyama and Katsuhiko Tsunekawa and Takumi Nagasawa and Michael Ploug and Ye Yang and Wenxin Song and Young, {Stephen G.} and Beigneux, {Anne P.} and Katsuyuki Nakajima and Masami Murakami",

year = "2024",

doi = "10.1016/j.jlr.2024.100532",

language = "English",

volume = "65",

journal = "Journal of Lipid Research",

issn = "0022-2275",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

number = "4",

}

RIS

TY - JOUR

T1 - Quantification of lipoprotein lipase in mouse plasma with a sandwich enzyme-linked immunosorbent assay

AU - Kimura, Takao

AU - Miyashita, Kazuya

AU - Fukamachi, Isamu

AU - Fukamachi, Kumiko

AU - Ogura, Kazumi

AU - Yokoyama, Erina

AU - Tsunekawa, Katsuhiko

AU - Nagasawa, Takumi

AU - Ploug, Michael

AU - Yang, Ye

AU - Song, Wenxin

AU - Young, Stephen G.

AU - Beigneux, Anne P.

AU - Nakajima, Katsuyuki

AU - Murakami, Masami

PY - 2024

Y1 - 2024

N2 - To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrations ranging from 0.31 ng/ml to 20 ng/ ml. The sensitivity of the ELISA made it possible to quantify LPL in serum and in both pre-heparin and post-heparin plasma samples (including in grossly lipemic samples). LPL mass and activity levels in the post-heparin plasma were lower in Gpihbp1¡/¡ mice than in wild-type mice. In both groups of mice, LPL mass and activity levels were positively correlated. Our mAb-based sandwich ELISA for mouse LPL will be useful for any investigator who uses mouse models to study LPL-mediated intravascular lipolysis.

AB - To support in vivo and in vitro studies of intravascular triglyceride metabolism in mice, we created rat monoclonal antibodies (mAbs) against mouse LPL. Two mAbs, mAbs 23A1 and 31A5, were used to develop a sandwich ELISA for mouse LPL. The detection of mouse LPL by the ELISA was linear in concentrations ranging from 0.31 ng/ml to 20 ng/ ml. The sensitivity of the ELISA made it possible to quantify LPL in serum and in both pre-heparin and post-heparin plasma samples (including in grossly lipemic samples). LPL mass and activity levels in the post-heparin plasma were lower in Gpihbp1¡/¡ mice than in wild-type mice. In both groups of mice, LPL mass and activity levels were positively correlated. Our mAb-based sandwich ELISA for mouse LPL will be useful for any investigator who uses mouse models to study LPL-mediated intravascular lipolysis.

KW - GPIHBP1

KW - HTGL

KW - lipids

KW - LPL

KW - transport

KW - triglycerides

KW - vascular biology

U2 - 10.1016/j.jlr.2024.100532

DO - 10.1016/j.jlr.2024.100532

M3 - Journal article

C2 - 38608546

AN - SCOPUS:85190612892

VL - 65

JO - Journal of Lipid Research

JF - Journal of Lipid Research

SN - 0022-2275

IS - 4

M1 - 100532

ER -

ID: 389550130

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