The murine receptor for urokinase-type plasminogen activator is primarily expressed in tissues actively undergoing remodeling
Research output: Contribution to journal › Journal article › Research › peer-review
uPAR is a cellular receptor for urokinase plasminogen activator, an enzyme involved in extracellular matrix degradation during processes involving tissue remodeling. We have expressed a recombinant soluble form of murine uPAR and raised rabbit polyclonal antibodies to study the expression of uPAR by immunohistochemistry. The immunohistochemical localization of uPAR was determined in normal mouse organs and in tumors formed by the highly metastatic Lewis lung carcinoma. uPAR immunoreactivity was found in the lungs, kidneys, and spleen, and in endothelial cells in the uterus, urinary bladder, thymus, heart, liver, and testis. No uPAR immunoreactivity was detected in muscle. In general, strong uPAR immunoreactivity was observed in organs undergoing extensive tissue remodeling, as exemplified by trophoblast cells in placenta, and in migrating, but not resting, keratinocytes at the edge of incisional wounds. Staining was not detected in any tissue sections derived from uPAR-deficient mice, thus confirming the specificity of the immunohistochemical staining of uPAR in normal mouse tissues. In Lewis lung carcinoma, uPAR immunoreactivity was found in the tumor cells of the primary tumor and in lung metastases. (J Histochem Cytochem 49:237-246, 2001)
Original language | English |
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Journal | Journal of Histochemistry and Cytochemistry |
Volume | 49 |
Issue number | 2 |
Pages (from-to) | 237-46 |
Number of pages | 10 |
ISSN | 0022-1554 |
DOIs | |
Publication status | Published - Feb 2001 |
Externally published | Yes |
- Animals, Antibodies, Blotting, Western, CHO Cells, Carcinoma, Lewis Lung, Cricetinae, Cross-Linking Reagents, Female, Immunohistochemistry, Lung Neoplasms, Mice, Mice, Inbred BALB C, Organ Specificity, Rabbits, Receptors, Cell Surface, Receptors, Urokinase Plasminogen Activator, Transfection, Urokinase-Type Plasminogen Activator, Journal Article, Research Support, Non-U.S. Gov't
Research areas
ID: 178216150