Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation: In memoriam Professor Niels Borregaard

Research output: Contribution to journal › Journal article › Research › peer-review

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Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation : In memoriam Professor Niels Borregaard. / Theilgaard-Mönch, Kim; Pundhir, Sachin; Reckzeh, Kristian; Su, Jinyu; Tapia, Marta; Furtwängler, Benjamin; Jendholm, Johan; Jakobsen, Janus Schou; Hasemann, Marie Sigurd; Knudsen, Kasper Jermiin; Cowland, Jack Bernard; Fossum, Anna; Schoof, Erwin; Schuster, Mikkel Bruhn; Porse, Bo T.

In: Nature Communications, Vol. 13, No. 1, 3595, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Theilgaard-Mönch, K, Pundhir, S, Reckzeh, K, Su, J, Tapia, M, Furtwängler, B, Jendholm, J, Jakobsen, JS, Hasemann, MS, Knudsen, KJ, Cowland, JB, Fossum, A, Schoof, E, Schuster, MB & Porse, BT 2022, 'Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation: In memoriam Professor Niels Borregaard', Nature Communications, vol. 13, no. 1, 3595. https://doi.org/10.1038/s41467-022-31332-1

APA

Theilgaard-Mönch, K., Pundhir, S., Reckzeh, K., Su, J., Tapia, M., Furtwängler, B., Jendholm, J., Jakobsen, J. S., Hasemann, M. S., Knudsen, K. J., Cowland, J. B., Fossum, A., Schoof, E., Schuster, M. B., & Porse, B. T. (2022). Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation: In memoriam Professor Niels Borregaard. Nature Communications, 13(1), [3595]. https://doi.org/10.1038/s41467-022-31332-1

Vancouver

Theilgaard-Mönch K, Pundhir S, Reckzeh K, Su J, Tapia M, Furtwängler B et al. Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation: In memoriam Professor Niels Borregaard. Nature Communications. 2022;13(1). 3595. https://doi.org/10.1038/s41467-022-31332-1

Author

Theilgaard-Mönch, Kim ; Pundhir, Sachin ; Reckzeh, Kristian ; Su, Jinyu ; Tapia, Marta ; Furtwängler, Benjamin ; Jendholm, Johan ; Jakobsen, Janus Schou ; Hasemann, Marie Sigurd ; Knudsen, Kasper Jermiin ; Cowland, Jack Bernard ; Fossum, Anna ; Schoof, Erwin ; Schuster, Mikkel Bruhn ; Porse, Bo T. / Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation : In memoriam Professor Niels Borregaard. In: Nature Communications. 2022 ; Vol. 13, No. 1.

Bibtex

@article{6aa8cf02052b4c52aebd2814dc904078,
title = "Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation: In memoriam Professor Niels Borregaard",
abstract = "Differentiation of multipotent stem cells into mature cells is fundamental for development and homeostasis of mammalian tissues, and requires the coordinated induction of lineage-specific transcriptional programs and cell cycle withdrawal. To understand the underlying regulatory mechanisms of this fundamental process, we investigated how the tissue-specific transcription factors, CEBPA and CEBPE, coordinate cell cycle exit and lineage-specification in vivo during granulocytic differentiation. We demonstrate that CEBPA promotes lineage-specification by launching an enhancer-primed differentiation program and direct activation of CEBPE expression. Subsequently, CEBPE confers promoter-driven cell cycle exit by sequential repression of MYC target gene expression at the G1/S transition and E2F-meditated G2/M gene expression, as well as by the up-regulation of Cdk1/2/4 inhibitors. Following cell cycle exit, CEBPE unleashes the CEBPA-primed differentiation program to generate mature granulocytes. These findings highlight how tissue-specific transcription factors coordinate cell cycle exit with differentiation through the use of distinct gene regulatory elements.",
author = "Kim Theilgaard-M{\"o}nch and Sachin Pundhir and Kristian Reckzeh and Jinyu Su and Marta Tapia and Benjamin Furtw{\"a}ngler and Johan Jendholm and Jakobsen, {Janus Schou} and Hasemann, {Marie Sigurd} and Knudsen, {Kasper Jermiin} and Cowland, {Jack Bernard} and Anna Fossum and Erwin Schoof and Schuster, {Mikkel Bruhn} and Porse, {Bo T}",
note = "{\textcopyright} 2022. The Author(s).",
year = "2022",
doi = "10.1038/s41467-022-31332-1",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Transcription factor-driven coordination of cell cycle exit and lineage-specification in vivo during granulocytic differentiation

T2 - In memoriam Professor Niels Borregaard

AU - Theilgaard-Mönch, Kim

AU - Pundhir, Sachin

AU - Reckzeh, Kristian

AU - Su, Jinyu

AU - Tapia, Marta

AU - Furtwängler, Benjamin

AU - Jendholm, Johan

AU - Jakobsen, Janus Schou

AU - Hasemann, Marie Sigurd

AU - Knudsen, Kasper Jermiin

AU - Cowland, Jack Bernard

AU - Fossum, Anna

AU - Schoof, Erwin

AU - Schuster, Mikkel Bruhn

AU - Porse, Bo T

N1 - © 2022. The Author(s).

PY - 2022

Y1 - 2022

N2 - Differentiation of multipotent stem cells into mature cells is fundamental for development and homeostasis of mammalian tissues, and requires the coordinated induction of lineage-specific transcriptional programs and cell cycle withdrawal. To understand the underlying regulatory mechanisms of this fundamental process, we investigated how the tissue-specific transcription factors, CEBPA and CEBPE, coordinate cell cycle exit and lineage-specification in vivo during granulocytic differentiation. We demonstrate that CEBPA promotes lineage-specification by launching an enhancer-primed differentiation program and direct activation of CEBPE expression. Subsequently, CEBPE confers promoter-driven cell cycle exit by sequential repression of MYC target gene expression at the G1/S transition and E2F-meditated G2/M gene expression, as well as by the up-regulation of Cdk1/2/4 inhibitors. Following cell cycle exit, CEBPE unleashes the CEBPA-primed differentiation program to generate mature granulocytes. These findings highlight how tissue-specific transcription factors coordinate cell cycle exit with differentiation through the use of distinct gene regulatory elements.

AB - Differentiation of multipotent stem cells into mature cells is fundamental for development and homeostasis of mammalian tissues, and requires the coordinated induction of lineage-specific transcriptional programs and cell cycle withdrawal. To understand the underlying regulatory mechanisms of this fundamental process, we investigated how the tissue-specific transcription factors, CEBPA and CEBPE, coordinate cell cycle exit and lineage-specification in vivo during granulocytic differentiation. We demonstrate that CEBPA promotes lineage-specification by launching an enhancer-primed differentiation program and direct activation of CEBPE expression. Subsequently, CEBPE confers promoter-driven cell cycle exit by sequential repression of MYC target gene expression at the G1/S transition and E2F-meditated G2/M gene expression, as well as by the up-regulation of Cdk1/2/4 inhibitors. Following cell cycle exit, CEBPE unleashes the CEBPA-primed differentiation program to generate mature granulocytes. These findings highlight how tissue-specific transcription factors coordinate cell cycle exit with differentiation through the use of distinct gene regulatory elements.

U2 - 10.1038/s41467-022-31332-1

DO - 10.1038/s41467-022-31332-1

M3 - Journal article

C2 - 35739121

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 3595

ER -

ID: 311509515