Deletion lengthening at chromosomes 6q and 16q targets multiple tumor suppressor genes and is associated with an increasingly poor prognosis in prostate cancer
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Deletion lengthening at chromosomes 6q and 16q targets multiple tumor suppressor genes and is associated with an increasingly poor prognosis in prostate cancer. / Kluth, Martina; Jung, Simon; Habib, Omar; Eshagzaiy, Mina; Heinl, Anna; Amschler, Nina; Masser, Sawinee; Mader, Malte; Runte, Frederic; Barow, Philipp; Frogh, Sohall; Omari, Jazan; Möller-Koop, Christina; Hube-Magg, Claudia; Weischenfeldt, Joachim; Korbel, Jan; Steurer, Stefan; Krech, Till; Huland, Hartwig; Graefen, Markus; Minner, Sarah; Sauter, Guido; Schlomm, Thorsten; Simon, Ronald.
In: OncoTarget, Vol. 8, No. 65, 12.12.2017, p. 108923-108935.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Deletion lengthening at chromosomes 6q and 16q targets multiple tumor suppressor genes and is associated with an increasingly poor prognosis in prostate cancer
AU - Kluth, Martina
AU - Jung, Simon
AU - Habib, Omar
AU - Eshagzaiy, Mina
AU - Heinl, Anna
AU - Amschler, Nina
AU - Masser, Sawinee
AU - Mader, Malte
AU - Runte, Frederic
AU - Barow, Philipp
AU - Frogh, Sohall
AU - Omari, Jazan
AU - Möller-Koop, Christina
AU - Hube-Magg, Claudia
AU - Weischenfeldt, Joachim
AU - Korbel, Jan
AU - Steurer, Stefan
AU - Krech, Till
AU - Huland, Hartwig
AU - Graefen, Markus
AU - Minner, Sarah
AU - Sauter, Guido
AU - Schlomm, Thorsten
AU - Simon, Ronald
PY - 2017/12/12
Y1 - 2017/12/12
N2 - Prostate cancer is characterized by recurrent deletions that can considerably vary in size. We hypothesized that large deletions develop from small deletions and that this "deletion lengthening" might have a "per se" carcinogenic role through a combinatorial effect of multiple down regulated genes.In vitroknockdown of 37 genes located inside the 6q12-q22 deletion region identified 4 genes with additive tumor suppressive effects, further supporting a role of the deletion size for cancer aggressiveness. Employing fluorescencein-situhybridization analysis on prostate cancer tissue microarrays, we determined the deletion size at 6q and 16q in more than 3,000 tumors. 16q and 6q deletion length was strongly linked to poor clinical outcome and this effect was even stronger if the length of both deletions was combined. To study deletion lengthening in cancer progression we eventually analyzed the entire cancers from 317 patients for 6q and 16q deletion length heterogeneity and found that the deletion expanded within 50-60% of 6q and 16q deleted cancers. Taken together, these data suggest continuous "deletion lengthening" as a key mechanism for prostate cancer progression leading to parallel down regulation of genes with tumor suppressive properties, some of which act cooperatively.
AB - Prostate cancer is characterized by recurrent deletions that can considerably vary in size. We hypothesized that large deletions develop from small deletions and that this "deletion lengthening" might have a "per se" carcinogenic role through a combinatorial effect of multiple down regulated genes.In vitroknockdown of 37 genes located inside the 6q12-q22 deletion region identified 4 genes with additive tumor suppressive effects, further supporting a role of the deletion size for cancer aggressiveness. Employing fluorescencein-situhybridization analysis on prostate cancer tissue microarrays, we determined the deletion size at 6q and 16q in more than 3,000 tumors. 16q and 6q deletion length was strongly linked to poor clinical outcome and this effect was even stronger if the length of both deletions was combined. To study deletion lengthening in cancer progression we eventually analyzed the entire cancers from 317 patients for 6q and 16q deletion length heterogeneity and found that the deletion expanded within 50-60% of 6q and 16q deleted cancers. Taken together, these data suggest continuous "deletion lengthening" as a key mechanism for prostate cancer progression leading to parallel down regulation of genes with tumor suppressive properties, some of which act cooperatively.
U2 - 10.18632/oncotarget.22408
DO - 10.18632/oncotarget.22408
M3 - Journal article
C2 - 29312579
VL - 8
SP - 108923
EP - 108935
JO - Oncotarget
JF - Oncotarget
SN - 1949-2553
IS - 65
ER -
ID: 193586222