Analysis of activated GAPs and GEFs in cell lysates
Research output: Contribution to journal › Journal article › Research › peer-review
An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.
| Original language | English |
|---|---|
| Book series | Methods in Enzymology |
| Volume | 406 |
| Pages (from-to) | 425-37 |
| Number of pages | 13 |
| ISSN | 0076-6879 |
| DOIs | |
| Publication status | Published - 2006 |
| Externally published | Yes |
- Animals, CHO Cells, Cricetinae, GTPase-Activating Proteins/metabolism, Glutathione Transferase/genetics, Guanine Nucleotide Exchange Factors/metabolism, Humans, Mice, NIH 3T3 Cells, Recombinant Fusion Proteins/metabolism, rho GTP-Binding Proteins/genetics
Research areas
ID: 199432674