TY - JOUR

T1 - Precision Cancer Medicine in the Acoustic Dispensing Era

T2 - Ex Vivo Primary Cell Drug Sensitivity Testing

AU - Kulesskiy, Evgeny

AU - Saarela, Jani

AU - Turunen, Laura

AU - Wennerberg, Krister

N1 - © 2015 Society for Laboratory Automation and Screening.

PY - 2016/2

Y1 - 2016/2

N2 - Cancer therapy is increasingly becoming individualized, but there are also big gaps between the molecular knowledge of individual cancers we can generate today and what can be applied in the clinic. In an attempt to bridge this knowledge gap between cancer genetic and molecular profiling and clinically useful information, an individualized systems medicine program has been established at the Institute for Molecular Medicine Finland (FIMM), University of Helsinki, and the Helsinki University Hospital. Central to this program is drug sensitivity and resistance testing (DSRT), in which responses of primary cancer cells to a comprehensive clinical oncology and signal transduction drug collection are monitored. The drug sensitivity information is used with molecular profiling to establish hypotheses on individual cancer-selective targeting drug combinations and their predictive biomarkers, which can be explored in the clinic. Here, we describe how acoustic droplet ejection is enabling DSRT in our cancer individualized systems medicine program to (1) generate consistent but configurable assay-ready plates and determine how this affects data quality, (2) flexibly prepare drug combination testing plates, (3) dispense reagents and cells to the assay plates, and (4) perform ultra-miniaturized follow-up assays on the cells from DSRT plates.

AB - Cancer therapy is increasingly becoming individualized, but there are also big gaps between the molecular knowledge of individual cancers we can generate today and what can be applied in the clinic. In an attempt to bridge this knowledge gap between cancer genetic and molecular profiling and clinically useful information, an individualized systems medicine program has been established at the Institute for Molecular Medicine Finland (FIMM), University of Helsinki, and the Helsinki University Hospital. Central to this program is drug sensitivity and resistance testing (DSRT), in which responses of primary cancer cells to a comprehensive clinical oncology and signal transduction drug collection are monitored. The drug sensitivity information is used with molecular profiling to establish hypotheses on individual cancer-selective targeting drug combinations and their predictive biomarkers, which can be explored in the clinic. Here, we describe how acoustic droplet ejection is enabling DSRT in our cancer individualized systems medicine program to (1) generate consistent but configurable assay-ready plates and determine how this affects data quality, (2) flexibly prepare drug combination testing plates, (3) dispense reagents and cells to the assay plates, and (4) perform ultra-miniaturized follow-up assays on the cells from DSRT plates.

KW - Acoustics

KW - Antineoplastic Agents/pharmacology

KW - Cell Survival/drug effects

KW - Cells, Cultured

KW - Cytological Techniques/methods

KW - Drug Resistance, Neoplasm

KW - Drug Screening Assays, Antitumor/methods

KW - Finland

KW - Hospitals, University

KW - Humans

KW - Neoplasms/drug therapy

KW - Precision Medicine/methods

U2 - 10.1177/2211068215618869

DO - 10.1177/2211068215618869

M3 - Journal article

C2 - 26721820

VL - 21

SP - 27

EP - 36

JO - SLAS TECHNOLOGY: Translating Life Sciences Innovation

JF - SLAS TECHNOLOGY: Translating Life Sciences Innovation

SN - 2211-0682

IS - 1

ER -