Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics. / Talwelkar, Sarang S.; Lähdeniemi, Iris A.K.; Mäyränpää, Mikko I.; Hemmes, Annabrita; Linnavirta, Nora; Räsänen, Jari; Knuuttila, Aija; Wennerberg, Krister; Verschuren, Emmy W.

In: STAR Protocols, Vol. 3, No. 4, 101720, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Talwelkar, SS, Lähdeniemi, IAK, Mäyränpää, MI, Hemmes, A, Linnavirta, N, Räsänen, J, Knuuttila, A, Wennerberg, K & Verschuren, EW 2022, 'Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics', STAR Protocols, vol. 3, no. 4, 101720. https://doi.org/10.1016/j.xpro.2022.101720

APA

Talwelkar, S. S., Lähdeniemi, I. A. K., Mäyränpää, M. I., Hemmes, A., Linnavirta, N., Räsänen, J., Knuuttila, A., Wennerberg, K., & Verschuren, E. W. (2022). Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics. STAR Protocols, 3(4), [101720]. https://doi.org/10.1016/j.xpro.2022.101720

Vancouver

Talwelkar SS, Lähdeniemi IAK, Mäyränpää MI, Hemmes A, Linnavirta N, Räsänen J et al. Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics. STAR Protocols. 2022;3(4). 101720. https://doi.org/10.1016/j.xpro.2022.101720

Author

Talwelkar, Sarang S. ; Lähdeniemi, Iris A.K. ; Mäyränpää, Mikko I. ; Hemmes, Annabrita ; Linnavirta, Nora ; Räsänen, Jari ; Knuuttila, Aija ; Wennerberg, Krister ; Verschuren, Emmy W. / Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics. In: STAR Protocols. 2022 ; Vol. 3, No. 4.

Bibtex

@article{c34e15c5c875422298150714d8dde3d4,
title = "Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics",
abstract = "Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021).",
keywords = "Cancer, Cell biology, Cell isolation, Cell-based assays, Clinical protocol, Health sciences, High throughput screening",
author = "Talwelkar, {Sarang S.} and L{\"a}hdeniemi, {Iris A.K.} and M{\"a}yr{\"a}np{\"a}{\"a}, {Mikko I.} and Annabrita Hemmes and Nora Linnavirta and Jari R{\"a}s{\"a}nen and Aija Knuuttila and Krister Wennerberg and Verschuren, {Emmy W.}",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.xpro.2022.101720",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Protocol to utilize fresh uncultured human lung tumor cells for personalized functional diagnostics

AU - Talwelkar, Sarang S.

AU - Lähdeniemi, Iris A.K.

AU - Mäyränpää, Mikko I.

AU - Hemmes, Annabrita

AU - Linnavirta, Nora

AU - Räsänen, Jari

AU - Knuuttila, Aija

AU - Wennerberg, Krister

AU - Verschuren, Emmy W.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021).

AB - Drug sensitivity data acquired from solid tumor-derived cultures are often unsuitable for personalized treatment guidance due to the lengthy turnaround time. Here, we present a protocol for determining ex vivo drug sensitivities using fresh uncultured human lung tumor-derived EpCAM+ epithelial cells (FUTCs). We describe steps for drug testing in FUTCs to identify tumor cell-selective single or combination therapy in 72 h of sample processing. The FUTC-based approach can also be used to predict in vivo resistance to known targeted therapies. For complete details on the use and execution of this protocol, please refer to Talwelkar et al. (2021).

KW - Cancer

KW - Cell biology

KW - Cell isolation

KW - Cell-based assays

KW - Clinical protocol

KW - Health sciences

KW - High throughput screening

U2 - 10.1016/j.xpro.2022.101720

DO - 10.1016/j.xpro.2022.101720

M3 - Journal article

C2 - 36170112

AN - SCOPUS:85138792963

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 4

M1 - 101720

ER -

ID: 322116536