Sequencing

The Sequencing Facility has an Illumina NextSeq500 sequencing machine, which has a single flow cell with four lanes. The NextSeq500 machine can run in two modes, a ”Hi” mode with longer run time (up to 30h) and an output of typically more than 400M reads, and a ”Mid” mode, with short run time and typically more than 140M reads.

Sequencing kits must be purchased via the facility. Read more about how to order here.

Sequencing libraries have to be prepared by the user and brought to the facility. Concentration of prepared sequencing libraries should be carefully measured. We recommend using QBit. Final concentration for optimal cluster generation depends on library type but are typically between 1 and 1.5pM.

Data is uploaded securely to both Illumina BaseSpace Sequence Hub and SUND IT, for local storage.

 

Training is available on request, depending on expected frequency of usage.

Only super-users are allowed to handle the machine (one of currently 12 super users can be contacted and can assist in running a flow cell).

Booking

To order/book a sequencing reagent kit, you have to register for a PPMS account at BRIC.

You can contact ngs_order@bric.ku.dk to inquire how to get this set up.

 

 

Prices for external users

Cat# Read_length Total time Output Reads Price DKK
FC-404-2002 2x75bp 18 hrs 50-60
Gb		 2x400M

24,125 DKK
FC-404-2005 1x75bp 11 hrs 25-30
Gb		 400M 12,550 DKK
FC-404-2001 2x75bp 15 hrs 16-20
Gb		 2x130M 9,370 DKK